Staphylococcus aureus|245563 |BNCC

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Micrococcus luteus (Schroeter) Cohn

Culture medium	BNCC363070
Description	Tryptic Soy Agar (TSA)
Composition	Tryptone:15.0g, Soy Peptone:5.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.2(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each Agar plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 1–2 mm in diameter, circular with entire margins, and opaque. The colony surface is golden-yellow, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram-positive (blue-violet) and spherical (cocci). The culture is pure.
Sharing mode	Public welfare sharing