Saccharomyces cerevisiae Sb48|245930 |BNCC

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Saccharomyces cerevisiae Hansen

Culture medium	BNCC352251
Description	YM agar medium
Composition	Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic;
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 2–4 mm in diameter, circular with entire margins, and opaque. The colony surface is milky white, convex, smooth, and moist in texture. They are easily picked up. The cells are Gram‑positive (blue‑violet) and ovoid. The culture is pure.
Sharing mode	Public welfare sharing