BeNa Culture Collection
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| Culture medium | BNCC384080 |
| Description | Acidic MRS Medium (1027) |
| Composition | Trypticase:10.0g, Beef extract:8.0g, Yeast extract:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, Triammonium citrate(C6H8O7.(NH4)3):2.0g, K2HPO4:2.0g, MnSO4:0.05g, Tween 80:1.0g, Agar:25.0g, pH:5.0 |
| Growth conditions | 30 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, yellow on the front, darker in color, flat in shape, smooth on the surface, moist in texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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