Fusobacterium nucleatum polymorphic subspecies|311817 |BNCC

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Fusobacterium nucleatum subsp. polymorphum (Knorr) Dzink et al.

Culture medium	BNCC353538
Description	Liquid thioglycolate culture medium
Composition	Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C₂H₃NaO₂S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃)
Growth conditions	37 ℃; 4-5 days; anaerobic;
Subculture procedure	① Prepare 1 tube of liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Fusobacterium nucleatum polymorphic subspecies, Gram negative non spore forming anaerobic bacterium, slender in shape with spindle like tips at both ends, turbid in thioglycolate liquid culture medium, liquid culture is recommended
Sharing mode	Public welfare sharing