BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30°C; 24-48h; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Open in a safety cabinet, cauterize the top with an alcohol lamp, then quickly add sterile water to break it open. Use forceps to crush it; ③ Pipette 0.5 mL sterile water into the freeze-dried vial. After fully dissolving the powder, dispense approximately 200 μL onto each of two plates and spread evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular, with neat, opaque edges; grayish-white upper surface; central elevation; smooth, moist texture; easily liftable; G+ (blue-violet); ovoid shape |
| Sharing mode | Public welfare sharing |
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