BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 18-24h; 5% CO2 |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colonies are 0.5–1 mm in diameter, circular with entire margins, and opaque. The colony surface is grayish-white, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram‑positive (blue‑violet) and rod‑shaped (bacilli). The culture is pure. |
| Sharing mode | Public welfare sharing |
Escherichia coli WM3064(Escherichia coli WM3064)
BNCC395800
Streptococcus sp. Grouping strain H60R(Streptococcus sp. Grouping strain H60R)
BNCC395802
Lactobacillus taiwanensis M9-1(Lactobacillus taiwanensis M9-1)
BNCC395799
Burkholderia pyrrocinia 2327(Burkholderia pyrrocinia 2327)
BNCC395783
Burkholderia cenocepacia YC40(Burkholderia cenocepacia YC40)
BNCC395737
Azotobactervinelandii(Azotobactervinelandii)
BNCC395776
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