Third Clostridium|187999 |BNCC

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Clostridium tertium

Culture medium	BNCC352241
Description	Columbia blood agar plate
Composition	Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	① Prepare 2 plates as described above (place them in an anaerobic environment for 24 hours for equilibration); ② Disinfect the surface of the ampoule tube and open it in a safety cabinet. Burn the top with an alcohol lamp, then quickly apply sterile water to crack it, followed by breaking it with tweezers; ③ Aspirate approximately 0.5 mL of liquid medium or sterile water (placed in an anaerobic environment for 24 hours for equilibration) into the freeze-dried tube, thoroughly dissolve the bacterial powder, and then inoculate onto 2 plates, 200 µL per Agar plate, spreading evenly; ④ Incubate the plates under the aforementioned culture conditions.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with serrated edges, opaque, gray white on the front, raised in the middle, smooth on the surface, bright on the surface, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing