Bifidobacterium bifidum|337499 |BNCC

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Bifidobacterium bifidum

Culture medium	BNCC352254
Description	BBL agar medium
Composition	Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃)
Growth conditions	37 ℃; 3-5 days; anaerobic
Subculture procedure	① Prepare a test tube containing 5-10 mL of liquid culture medium; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube; ④ Cultivate the liquid test tube under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	Bifidobacterium bifidum, a Gram positive bacterium, is polymorphic. The BBL liquid culture medium is turbid, and the growth of the Agar plate strain is weak. It is recommended to use liquid culture
Sharing mode	Public welfare sharing