Imitate Staphylococcus|340329 |BNCC

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Staphylococcus simulans

Culture medium	BNCC369667
Description	Nutrient Broth (NB)
Composition	Peptone:10.0g, Beef powder:3.0g, NaCl:5.0g, pH:7.2±0.2(25℃)
Growth conditions	37 ℃; 18-24 hours; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium or sterile water and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colonies are 1–2 mm in diameter, circular with entire margins, and opaque. The colony surface is white, convex, shiny, smooth, and moist in texture. They are easily picked up. The cells are Gram-positive (blue-violet) and spherical (cocci). The culture is pure.
Sharing mode	Public welfare sharing