BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC352252 |
| Description | Brain Heart Infusion Agar |
| Composition | Beef Brain Infusion Powder:4.0g, Beef Heart Infusion Powder:4.0g, Peptone:5.0g, Casein Peptone:16.0g, NaCl:5.0g, Glucose:2.0g, Na2HPO4:2.5g, Agar:13.5g, pH:7.4±0.2(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic; |
| Subculture procedure | ① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front and gray white on the back, protruding in the middle, smooth on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus subtilis |
| Sharing mode | Public welfare sharing |
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