BeNa Culture Collection
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| Culture medium | BNCC352254 |
| Description | BBL agar medium |
| Composition | Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃) |
| Growth conditions | 37 ℃; 48-72 hours; anaerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colonies are 1–2 mm in diameter, circular with entire margins, and opaque. The colony surface is cream-white, convex, smooth, shiny, and moist in texture. They are easily picked up. The cells are Gram-positive (blue-violet) and rod-shaped (bacilli). The culture is pure. |
| Sharing mode | Public welfare sharing |
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