Bacillus cereus|353904 |BNCC

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Sporosarcina pasteurii (Miquel 1889) Yoon et al. 2001

Culture medium	BNCC351989
Description	Soybean Casein Digest Agar with Urea
Composition	Peptone:5.0g, Beef Extract:3.0g, Urea:20.0g, Agar:15.0g, pH:7.3(25℃)
Growth conditions	30 ℃; 24-48 hours; aerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing