Fecal Roseburiella|354450 |BNCC

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Roseburia faecis

Culture medium	BNCC376181
Description	Modified YCFA Medium Base
Composition	Trypticase:10.0g, Yeast extract:2.5g, Glucose:5g, K₂HPO₄:0.45g, KH₂PO₄:0.45g, NaCl:0.9g, MgSO₄.7H₂O:45.00mg, CaCl₂.2H₂O:90.00mg, Resazurin:1.0mg, Volatile fatty acids:10ml, NaHCO₃:4.0g, Cysteine hydrochloride(C₃H₇NO₂S.HCl):1.0g, Hemin(C₃₄H₃₂ClFeN₄O₄):10.0mg, Vitamin solution:10ml
Growth conditions	37 ° C; 24-48 hours; anaerobic
Subculture procedure	① Dissolve the Frozen vial in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into one test tube (the culture medium needs to be deoxygenated 24 hours in advance); ④ After uniform coating, transfer to the above cultivation conditions for cultivation.
Storage conditions	-80 ℃
Safety level	1
morphology	G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing