BeNa Culture Collection
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| Culture medium | BNCC376181 |
| Description | Modified YCFA Medium Base |
| Composition | Trypticase:10.0g, Yeast extract:2.5g, Glucose:5g, K2HPO4:0.45g, KH2PO4:0.45g, NaCl:0.9g, MgSO4.7H2O:45.00mg, CaCl2.2H2O:90.00mg, Resazurin:1.0mg, Volatile fatty acids:10ml, NaHCO3:4.0g, Cysteine hydrochloride(C3H7NO2S.HCl):1.0g, Hemin(C34H32ClFeN4O4):10.0mg, Vitamin solution:10ml |
| Growth conditions | 37 ° C; 24-48 hours; anaerobic |
| Subculture procedure | ① Dissolve the Frozen vial in a 37 ℃ water bath and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to an anaerobic glove box for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into one test tube (the culture medium needs to be deoxygenated 24 hours in advance); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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