BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ° C; 2-3 days; aerobic; |
| Subculture procedure | ① Dissolve the cryovial in a water bath (mold at 30 ℃) and shake rapidly to dissolve; ② Wipe the outer wall of the cryovial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates; ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | Liquid nitrogen |
| Safety level | 1 |
| morphology | Small filamentous fungi, with obvious colonies on comprehensive PDA medium, black color, dense and vigorous hyphae, spreading and growing towards the edge of the Agar plate, |
| Sharing mode | Public welfare sharing |
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