BeNa Culture Collection
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| Culture medium | BNCC352255 |
| Description | Streptomyces medium 2 |
| Composition | Yeast Extract Powder:4.0g, Malt Extract Powder:10.0g, Glucose:4.0g, Agar:20.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 45 ℃; 3-5 days; aerobic; |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colonies are 2–4 mm in diameter, circular with irregular margins, and opaque. The colony surface is yellowish-brown, convex, rough, dull, and dry in texture. The cells are Gram‑positive (blue‑violet) and rod‑shaped (bacilli), with old hyphae fragmenting into bacilloid or coccoid forms. The culture is pure. |
| Sharing mode | Public welfare sharing |
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