Fecal anaerobic bacteria|367912 |BNCC

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Anaerostipes faecis

Culture medium	BNCC353538
Description	Liquid thioglycolate culture medium
Composition	Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C₂H₃NaO₂S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃)
Growth conditions	37 ℃; 3-4 days; anaerobic;
Subculture procedure	Prepare a liquid culture medium with a diameter of 18mm, approximately 10mL (placed in an anaerobic environment for 24 hours of deoxygenation); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Transfer approximately 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back to the liquid culture medium in the test tube; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, does not produce pigments, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing