BeNa Culture Collection
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| Culture medium | BNCC352251 |
| Description | YM agar medium |
| Composition | Yeast Extract Powder:3.0g, Malt Extract:3.0g, Glucose:10.0g, Casein Peptone:5.0g, Agar:20.0g, pH:6.2±0.2(25℃) |
| Growth conditions | 30 ℃; 18-24h; aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colonies measure 2–4 mm in diameter, are circular with entire margins, opaque, and cream-colored (milky white). They exhibit a convex elevation with a smooth, glistening surface and a moist, viscous texture. |
| Sharing mode | Public welfare sharing |
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