Dermatitis bottle fungi|360135 |BNCC

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Exophiala dermatitidis

Culture medium	BNCC350785
Description	Sabouraud glucose agar
Composition	Glucose:40.0g, Peptone:10.0g, Agar:15.0g, pH:5.6±0.2(25℃)
Growth conditions	30 °C; 24-48 hours; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, gray on the front, raised in the middle, and gray on the surface. It is G+(blue purple) and cocci
Sharing mode	Public welfare sharing