BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; Aerobic |
| Subculture procedure | ① Prepare one 90mm solid plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the plate; ⑤ Place the flat plate in the mold incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The plate can be used when it is almost full. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungi have obvious colonies on comprehensive PDA medium, with white hyphae in the early stage, dense and vigorous hyphae, spreading and growing towards the edge of the plate, and a light pink fungal layer in the later stage |
| Sharing mode | Public welfare sharing |
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