Fusarium oxysporum f. sp. cucumerinum J.H. Owen|116160 |BNCC

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Fusarium oxysporum f.sp. cucumerinum J.H. Owen

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃; 5-7 days; aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the agar plate and onto the surface of the agar; ⑤ Cultivate the flat agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	A small filamentous fungus forms distinct colonies on general PDA medium. The colonies are white with dense, low-lying mycelium. The reverse side of the agar plate shows a pale yellow pigmentation.
Sharing mode	Public welfare sharing