Mycobacterium abscesses|370010 |BNCC

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Mycobacterium abscessus

Culture medium	BNCC362337
Description	Chocolate Agar Plate
Composition	Polypeptone:18.0g, Starch:1.0g, NaCl:5.0g, Agar:15.0g, Defibrinated Sheep Blood:40mL, pH:7.4±0.2(25℃)
Growth conditions	37 ℃; 48-72h；5%CO2；
Subculture procedure	① Prepare 1-2 of the above plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony has a diameter of 1-2mm, spreads and grows irregularly, with irregular shapes and edges, is opaque, gray white on the front, flat in shape, rough on the surface, wrinkled on the surface, dry in texture, G+(blue purple), rod-shaped, purity: pure
Sharing mode	Public welfare sharing