BeNa Culture Collection
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| Subculture procedure | ① Dissolve the Frozen vial in a 37 ℃ water bath, and shake quickly during the dissolution process; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen, with each Agar plate containing approximately 200 μ L); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Growth conditions | 37 ° C; 3-7 days; Strictly anaerobic |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with irregular edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |