BeNa Culture Collection
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| Culture medium | BNCC370536 |
| Description | Sabouraud Dextrose Agar (SDA) |
| Composition | Glucose:40.0g, Peptone:10.0g, Agar:20.0g, pH:5.6±0.2(25℃) |
| Growth conditions | 30 ℃; 48-72h; aerobic; |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, round, with neat edges, opaque, milky white on the front, raised in the middle, bright and smooth on the surface in the early stage, wrinkled on the surface in the later stage, sticky texture, G+(blue purple), oval, purity: pure |
| Sharing mode | Public welfare sharing |
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