BeNa Culture Collection
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| Culture medium | BNCC362653 |
| Description | GYM Streptomyces Agar |
| Composition | Glucose:4.0g, Yeast Extract:4.0g, Malt Extract:10.0g, CaCO3:2.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 28 ℃; 3-5 days; aerobic; |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front and yellow on the back, protruding in the middle, hard and difficult to pick, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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