BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 25-28 ℃, 5-7 days, aerobic |
| Subculture procedure | ① Prepare one 90mm solid Agar plate, one inoculation shovel and one inoculation hoe; ② Inoculation shovel, inoculation hoe, flame burn, cool for later use; ③ After disinfecting the outer wall of the inclined surface, open it in a sterile environment, first use an inoculation hoe, and then use an inoculation shovel to cut into pieces; ④ Inoculate the bacterial block with a spatula, place it on the surface of a agar plate, and cover the Agar plate; ⑤ Place the flat Agar plate in the fungi incubator and cultivate it according to the above conditions. Do not seal it with a sealing film. The Agar plate can be used when it is almost full. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The isolate was a small filamentous fungus. On general PDA medium, it formed distinct, white colonies with dense and vigorous mycelia, exhibiting spreading growth toward the Agar plate edges. The reverse side of the medium appeared grayish-white. |
| Sharing mode | Public welfare sharing |
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