Propionibacterium|378111 |BNCC

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Arachnia propionica

Culture medium	BNCC360852
Description	EG Medium
Composition	Beef Extract Powder:2.4g, Proteose Peptone:10.0g, Yeast Extract Powder:5.0g, Na₂HPO₄:4.0g, Glucose:1.5g, Soluble Starch:0.5g, L-Cystine:0.2g, Agar:15.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, Sterile Horse Blood:50mL, pH:7.6-7.8(25℃)
Growth conditions	37 ℃; 3-4 days; anaerobic;
Subculture procedure	Prepare 1-2 of the above plates (placed in an anaerobic environment for 24 hours of deoxygenation in advance) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, bright and smooth on the surface, moist texture, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing