BeNa Culture Collection
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| Culture medium | BNCC360852 |
| Description | EG Medium |
| Composition | Beef Extract Powder:2.4g, Proteose Peptone:10.0g, Yeast Extract Powder:5.0g, Na2HPO4:4.0g, Glucose:1.5g, Soluble Starch:0.5g, L-Cystine:0.2g, Agar:15.0g, L-Cysteine Hydrochloride(C3H7NO2S.HCl):0.5g, Sterile Horse Blood:50mL, pH:7.6-7.8(25℃) |
| Growth conditions | 37 ℃; 48-72 hours; anaerobic |
| Subculture procedure | Prepare 1-2 plates and sterile water (pre placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth on the surface, moist in texture, does not produce pigments, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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