Fecal black egg nest fungus|122317 |BNCC

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Cyathus stercoreus (Schwein.) De Toni

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃, 5-7 days, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	2-8 ℃
Safety level	1
morphology	A small filamentous fungus forming distinct colonies on general PDA. The colony consists exclusively of white, spreading (radial) mycelium that grows vigorously towards the Agar plate margin. Under these cultural conditions, the isolate does not produce any spores (sporulation was not observed).
Sharing mode	Public welfare sharing