Fusarium graminearum|124121 |BNCC

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Gibberella intricans Wollenw.

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	25~28 ℃, 3-5 days, aerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	-80 ℃
Safety level	1
morphology	On general Potato Dextrose Agar (PDA), this small filamentous fungus forms distinct colonies. The colonies are white, consisting of dense and vigorous mycelium that exhibits spreading growth toward the Agar plate margins. A light yellow pigmentation is observed on the reverse side of the medium.
Sharing mode	Public welfare sharing