Matsutake mushrooms|124452 |BNCC

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Tricholoma matsutake (S. Ito & S. Imai) Singer

Culture medium	BNCC352250
Description	Comprehensive PDA agar
Composition	Potato Infusion Powder:10.0g, Glucose:20.0g, KH₂PO₄:3.0g, MgSO₄.7H₂O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃)
Growth conditions	28 ℃, 1-2 months, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the Agar plate and onto the surface of the agar; ⑤ Cultivate the flat Agar plate under the above cultivation conditions, and the strain can be used once it grows.
Storage conditions	-80 ℃
Safety level	1
morphology	On general Potato Dextrose Agar (PDA), this small filamentous fungus forms distinct colonies. The colonies are off-white in color and exhibit a spreading growth pattern toward the Agar plate margins, though growth is slow. The reverse side of the medium shows a pale yellow pigmentation.
Sharing mode	Public welfare sharing