Bacillus subtilis|132668 |BNCC

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Bacillus megaterium

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	The isolate is a pure culture of Gram‑positive (blue‑violet) bacilli. It forms circular, opaque, convex colonies (1–2 mm) with entire margins, a shiny, smooth, grayish‑white surface, and a moist texture, allowing for easy removal.
Sharing mode	Public welfare sharing