BeNa Culture Collection
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| Culture medium | BNCC360917 |
| Description | Penassay Agar |
| Composition | Beef Extract:1.5g, Yeast Extract:1.5g, Tryptone:5.0g, Glucose:1.0g, NaCl:3.5g, K2HPO4:4.8g, KH2PO4:1.32g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The isolate forms small (0.5–1 mm), circular, opaque, flat colonies with entire margins, a bright, smooth, pale yellow surface, and is easily removable. Microscopic examination reveals Gram‑positive (blue‑violet) bacilli. |
| Sharing mode | Public welfare sharing |
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