BeNa Culture Collection
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| Culture medium | BNCC360815 |
| Description | Nitrogen fixing medium |
| Composition | KH2PO4:0.2g, K2HPO4:0.8g, MgSO4.7H2O:0.2g, CaSO4.2H2O:0.1g, FeCl3:Trace, Na2MoO4.2H2O:Trace, Yeast Extract:0.5g, Mannitol:20.0g, Agar:15.0g, pH:7.2(25℃) |
| Growth conditions | 30 ℃; 48-72h; aerobic |
| Subculture procedure | ① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colonies measure 1-2 mm in diameter, are circular with regular margins, and are opaque. They exhibit a grayish-white pigmentation on the surface, have a raised center, and are smooth, glossy, and moist in texture, making them easy to lift. The organisms are Gram-negative (G⁻, appearing red) and rod-shaped (bacilli). Purity: pure. |
| Sharing mode | Public welfare sharing |
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