BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360926 |
| Description | M17 Agar |
| Composition | Soy Peptone:5.0g, Beef Peptone:2.5g, Casein Peptone:2.5g, Yeast Extract Powder:2.5g, Meat Infusion Powder:5.0g, Lactose:5.0g, Sodium Ascorbate(C6H7NaO6):0.5g, β-Glycerophosphate Sodium(C3H7Na2O6P):19.0g, MgSO4:0.25g, Agar:12.75g, pH:7.2±0.2(25℃) |
| Growth conditions | 37 ℃; 3-5 days; anaerobic |
| Subculture procedure | ① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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