Streptococcus thermophilus|232113 |BNCC

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Streptococcus thermophilus

Culture medium	BNCC360926
Description	M17 Agar
Composition	Soy Peptone:5.0g, Beef Peptone:2.5g, Casein Peptone:2.5g, Yeast Extract Powder:2.5g, Meat Infusion Powder:5.0g, Lactose:5.0g, Sodium Ascorbate(C₆H₇NaO₆):0.5g, β-Glycerophosphate Sodium(C₃H₇Na₂O₆P):19.0g, MgSO₄:0.25g, Agar:12.75g, pH:7.2±0.2(25℃)
Growth conditions	37 ℃; 3-5 days; anaerobic
Subculture procedure	① Prepare 2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water (placed in an anaerobic environment for 24 hours) and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into 2 plates at a rate of 200 μ L per Agar plate and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 1-2mm, circular, with neat edges, opaque, milky white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), cocci, purity: pure
Sharing mode	Public welfare sharing