Clostridium|185375 |BNCC

Essential Information Related Products

Clostridium sp.

Culture medium	BNCC353538
Description	Liquid thioglycolate culture medium
Composition	Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C₂H₃NaO₂S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃)
Growth conditions	37 ℃; 24-48 hours; anaerobic;
Subculture procedure	① Prepare one 18mm test tube liquid culture medium, approximately 10mL (placed in anaerobic environment for 24 hours of deoxygenation in advance); ② Disinfect the surface of the ampoule tube, open it in a safe cabinet, burn the top with an alcohol lamp, quickly add sterile water to the burning area to break it, and then use tweezers to knock it open; ③ Take about 0.5mL of liquid culture medium and transfer it into a freeze-drying tube. After fully dissolving, take it back into the liquid culture medium in the test tube and mix well; ④ Place the liquid test tube under the specified "culture conditions" mentioned above for cultivation. If the bacterial liquid is turbid or there is a large amount of bacterial growth at the bottom, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Clostridium beijerinckii is an anaerobic bacterium. Microscopic examination revealed Gram-positive rods. After 48 hours of cultivation in FT medium, a significant amount of sediment was observed at the bottom of the test tube, accompanied by gas production.Growth of the organism is also supported on ready-to-use Columbia blood agar plates under anaerobic conditions.
Sharing mode	Public welfare sharing