Hooked Agrobacterium|134955 |BNCC

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Agrobacterium rubi

Culture medium	BNCC353644
Description	Rhizobium Medium 1
Composition	K₂HPO₄:0.5g, MgSO₄.7H₂O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions	30 ℃; 18-24h; aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all liquid test tubes and plates under the above cultivation conditions, and the bacterial strains can be used once they grow.
Storage conditions	2-8 ℃
Safety level	1
morphology	The bacterium forms small (0.5–1 mm), circular, opaque, convex colonies with entire margins, a smooth, light red surface, and a moist texture. Microscopic examination reveals Gram‑negative (red) bacilli.
Sharing mode	Public welfare sharing