Bacillus subtilis|233504 |BNCC

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Bacillus subtilis (Ehrenberg) Cohn

Culture medium	BNCC352239
Description	Nutrient Agar
Composition	Peptone:10.0g, Beef Extract Powder:3.0g, NaCl:5.0g, Agar:15.0g, pH:7.3±0.1(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare one test tube containing 5-10 mL of liquid culture medium and two plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Test the liquid
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, irregular in shape, irregular in edges, opaque, gray white on the front, flat in shape, rough on the surface, dry in texture, G+(blue purple), rod-shaped
Sharing mode	Public welfare sharing