Non symbiotic luminescent bacteria|233870 |BNCC

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Photorhabdus asymbiotica subsp.asymbiotica Fischer-Le Saux et al.3265-86

Culture medium	BNCC352232
Description	Tryptic Soy Broth
Composition	Tryptone:17.0g, Soy Peptone:3.0g, NaCl:5.0g, K₂HPO₄:2.5g, Glucose:2.5g, pH:7.3±0.2(25℃)
Growth conditions	30 ℃, 18-24h, aerobic
Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	2
morphology	The colony diameter is 2-4mm, circular, with neat edges, opaque, yellow on the front and yellow on the back, protruding in the middle, smooth and bright on the surface, moist texture, easy to pick up, G - (red), Bacillus, purity: pure
Sharing mode	Public welfare sharing