BeNa Culture Collection
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| Culture medium | BNCC352254 |
| Description | BBL agar medium |
| Composition | Peptone:15.0g, Glucose:20.0g, Yeast Extract Powder:2.0g, Soluble Starch:0.5g, NaCl:5.0g, L-Cysteine:0.5g, Tomato Infusion Powder:5.0g, Liver Infusion Powder:2.0g, Tween 80:1.0mL, Agar:20.0g, pH:7.0±0.1(25℃) |
| Growth conditions | 37 ℃; 48-72h; anaerobic; |
| Subculture procedure | ① Dissolve the frozen tube in a water bath (37 ℃ for bacteria) and shake quickly to dissolve; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into 1-2 agar plates (each Agar plate contains approximately 200 μ L, and for anaerobic bacteria cultivation, the culture medium needs to be placed in an anaerobic environment for 24 hours to remove oxygen); ④ After uniform coating, transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Colonies measured 1-2 mm in diameter, appearing circular with an entire margin and opaque. They were white in color, convex in elevation, with a smooth, moist, and easily liftable texture. Microscopic examination revealed Gram-positive (staining blue-violet) bacilli. |
| Sharing mode | Public welfare sharing |
UACC-812(UACC-812)
BNCC338200
H22(H22)
BNCC338327
HCC-78(HCC-78)
BNCC338064
NCI-H1373(NCI-H1373)
BNCC341744
HepAD38(HepAD38)
BNCC341745
NCI-H1693(NCI-H1693)
BNCC342281