BeNa Culture Collection
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| Culture medium | BNCC370433 |
| Description | de Man, Rogosa and Sharpe (MRS) Broth |
| Composition | Peptone:10.0g, Beef extract:8.0g, Yeast powder:4.0g, Glucose:20.0g, MgSO4:0.2g, CH3COONa:5.0g, (NH4)2HC6H5O7:2.0g, K2HPO4:2.0g, MnSO4:0.04g, Tween 80:1.0g, pH:5.7±0.2(25℃) |
| Growth conditions | 37 ℃; 24-48 hours; aerobic |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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