BeNa Culture Collection
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| Culture medium | BNCC360812 |
| Description | Concentrated sugar Chatelier agar |
| Composition | Sucrose:200.0g, NaNO3:3.0g, MgSO4.7H2O:0.5g, KCl:0.5g, FeSO4.4H2O:0.01g, K2HPO4:1.0g, Agar:15.0g, pH:6.0-6.5(25℃) |
| Growth conditions | 25 ℃, 14-20 days, aerobic |
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | Spread and grow on concentrated sugar Chaetoceros, with white mycelium in the early stage and yellow spores in the later stage |
| Sharing mode | Public welfare sharing |
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