Brown ball nitrogen fixing bacteria|137688 |BNCC

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Azotobacter chroococcum

Culture medium	BNCC353644
Description	Rhizobium Medium 1
Composition	K₂HPO₄:0.5g, MgSO₄.7H₂O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃)
Growth conditions	28 ℃; 48-72h; aerobic;
Subculture procedure	① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, semi transparent, light pink color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), cocci, purity: pure
Sharing mode	Public welfare sharing