BeNa Culture Collection
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| Culture medium | BNCC353644 |
| Description | Rhizobium Medium 1 |
| Composition | K2HPO4:0.5g, MgSO4.7H2O:0.2g, NaCl:0.1g, Yeast Extract:1.0g, Mannitol:10.0g, Congo Red:0.025g, Agar:15.0g, pH:7.2±0.2(25℃) |
| Growth conditions | 28 ℃; 48-72h; aerobic; |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, semi transparent, light pink color, raised in the middle, smooth surface, bright surface, moist texture, easy to pick up, G - (red), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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