Streptococcus equi subsp. zooepidemicus|281388 |BNCC

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Streptococcus equi subsp. zooepidemicus Farrow and Collins

Subculture procedure	① Prepare 1-2 of the above plates; ② After disinfecting the surface of the ampoule tube, open it in a safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into a Agar plate at a rate of 200 μ L per piece and apply evenly; ④ Place the petri dish under the above cultivation conditions for 18-24 hours.
Growth conditions	37 ℃, 18-24h, aerobic
Storage conditions	2-8 ℃
Safety level	2
morphology	Size: 2-4mm Shape: Irregular Edge: Neat Transparency: Translucent Color: Grey White Uplift Degree: Middle Raised Surface: Bright and Smooth Texture: Wet and Viscous
Sharing mode	Public welfare sharing

Description

1. Name: HA-116 Streptococcus equi subsp. zooepidemicus Farrow and Collins

2. BNCC No.: 281388

3. Biosafety level:3

2. Storage conditions:

Storage of freezed dried ampoule and agar slant at 2°C to 8°C

3. Growth Conditions

1, TSA/TSB (tryptone soybean medium): tryptone 17.0g, soy peptone 3.0g, sodium chloride 5.0g, dipotassium hydrogen phosphate 2.5g, glucose 2.5g, agar 20.0g (not included in liquid medium), distilled water 1.0L,pH 7.3±0.2. Sterilization at 121 ℃ for 15min.

2. Atmosphere: aerobic

3. Temperature: 37 ℃

4. Notes:
1.Normal culturing time, 24-48hours for bacterial, 72 hours for yeast, 5-7days for mould, 7-10days for fungal.

2.Agar slant shall be inoculated asap, and do not keep the storage for more than 3 months.

3.Please recover the strains in strict accordance with this instruction, otherwise the replacement of the strain are not be available in case of viability loss caused by different media or growth conditions.

4.Waste generated from the handling process should be discarded after high-pressure sterilization

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