Wolbachia cholerae|358991 |BNCC

Essential Information Certificate Related Products

Bilophila wadsworthia

Subculture procedure	①Prepare 1-2 fresh agar plates (placed in an anaerobic environment for 24 hours of deoxygenation) with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of liquid culture medium or sterile water (placed in an anaerobic environment for 24 hours of deoxygenation in advance) and transfer it into a freeze-drying tube. Dissolve it thoroughly and mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows.
Growth conditions	37 ℃; 3-4 days; anaerobic;
Storage conditions	2-8 ℃
Safety level	1
morphology	The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth and bright on the surface, moist texture, easy to pick up, G+(blue purple), Bacillus, purity: pure
Sharing mode	Public welfare sharing

Bilophila wadsworthia

Storage conditions: 2~8 ℃

No. 358991

Product format: freeze dried,200ul

Validity period: 6 years

Biosafety level: 1, handle in ultra-clear table or safety cabinet

Receiving notice: if any abnormality is found on the day of receipt, please contact the customer service within 24 hours. If it is overdue, it is deemed that the bacteria are well. The freeze dried culture shall be used up once and shall not be retained. The bacterial viability will resume after 1-2 generation of recovery and can be used normally. Handling instructions of agar slant and suspension liquid is enclosed. Please operate in strict accordance with this instruction, otherwise the replacement of bacteria are not be available in case of aberrant growth and loss of viability.

Growth conditions:37 ℃, strictly anaerobic, Colombian blood plate (ready-to-use type),3-4 days.

Recovery steps:

①Prepare 1-2 pieces of Blood agar plates;

②sterilizing the ampoule, open it in biosafety cabinet, heat the tip of ampoule in a flame, quickly drop sterile water to creak it, then break it with forceps;

③draw 0.5mL of liquid culture medium into the freeze dried ampoule,make the strain pellet fully dissolved and inoculate a plate with the solution, then distribute it well in 200ml/plate;

④Put the agar plates under the above culture conditions for cultivation.

Recovery record: According to the recovery instructions, the results of the recovery are reported as follows:

Item	test results
viability:	good viability. in 72 hour obvious strain layer occurs on the plate; colony is typical
Colony morphology: (pictured above)	size: 1-2mm ; Shape: round ; Edge: irregular ; Transparency: opaque ; Color: off-white uplift: middle bulge ; Surface: gray ; Texture: moist and easy to stir
conclusion: good viability, no abnormal colony morphology, qualified

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