Animal Bifidobacterium subspecies|256597 |BNCC

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Bifidobacterium animalis subsp.‖lactis

Culture medium	BNCC360803
Description	Enhanced Clostridium agar medium
Composition	Yeast Extract:3.0g, Beef Extract Powder:10.0g, Glucose:5.0g, Pancreatic Casein Peptone:10.0g, NaCl:5.0g, Soluble Starch:1.0g, L-Cysteine Hydrochloride(C₃H₇NO₂S.HCl):0.5g, CH₃COONa:3.0g, Agar:12.5g, pH:6.8±0.2(25℃)
Growth conditions	37 ℃, 24-48 hours, anaerobic
Subculture procedure	① Prepare 1-2 tablets mentioned above; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and transfer it into a freeze-drying tube. After fully dissolving the bacterial powder, transfer it into two plates, each plate containing about 200 μ L, and apply evenly; ④ Place the petri dish under the above cultivation conditions for cultivation, and once the bacterial strain grows, it can be used.
Storage conditions	2-8 ℃
Safety level	1
morphology	Size: 1-2mm Shape: Irregular Edge: Neat Transparency: Opaque Color: Milky White Rise Degree: Middle Rise Surface: Bright and Smooth Texture: Wet and Viscous
Sharing mode	Public welfare sharing