BeNa Culture Collection
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| Culture medium | BNCC352241 |
| Description | Columbia blood agar plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:10.0g, Defibrinated Sheep Blood:5%(added at 50℃), pH:7.3±0.2(25℃) |
| Growth conditions | 37°C; 18-24h; 5% CO₂ |
| Subculture procedure | ① Prepare 1-2 plates as above; ② Disinfect ampoule surface, open in safety cabinet, cauterize top with alcohol lamp, then immediately break with sterile water; shatter contents with forceps; ③ Pipette 0.5mL sterile water into lyophilized tube, dissolve powder completely, then dispense 200μL/well onto plates, spreading evenly; ④ Incubate plates under specified conditions until colonies emerge. |
| Storage conditions | 2-8°C |
| Safety level | 1 |
| morphology | Colony diameter: 1-2 mm; circular shape with neat margins; grayish-white upper surface; central convexity; smooth texture; moist consistency; non-pigmented; Gram-negative (red); bacillus; purity: pure |
| Sharing mode | Public welfare sharing |
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