BeNa Culture Collection
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| Culture medium | BNCC352250 |
| Description | Comprehensive PDA agar |
| Composition | Potato Infusion Powder:10.0g, Glucose:20.0g, KH2PO4:3.0g, MgSO4.7H2O:1.5g, Thiamine:0.008g, Agar:15.0g, pH:6.0±0.2(25℃) |
| Growth conditions | 28 ℃; 5-7 days; aerobic |
| Subculture procedure | ① Prepare 1-2 plates as mentioned above; ② After disinfecting the inclined surface of the test tube, open it in the safety cabinet; ③ Cut small square blocks of 0.5 × 0.5cm2 using a sterile inoculation hoe and inoculation shovel (see attached page); ④ Place the small square block flat on the center of the agar plate and onto the surface of the agar; ⑤ Cultivate the flat agar plate under the above cultivation conditions, and the strain can be used once it grows. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | Small filamentous fungus, exhibiting distinct colonies on comprehensive PDA medium, grayish-brown in color, with dense and vigorous mycelia spreading towards the edges of the agar plate; the reverse side of the medium appears pale yellow. |
| Sharing mode | Public welfare sharing |
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