BeNa Culture Collection
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| Culture medium | BNCC353703 |
| Description | 2216E agar |
| Composition | Peptone:5.0g, Yeast Extract:1.0g, Ferric Citrate(FeC6H5O7):0.1g, KBr:0.08g, NaCl:19.45g, MgCl2:5.98g, Na2SO4:3.24g, CaCl2:1.8g, KCl:0.55g, Na2CO3:0.16g, Na2NO3:0.0016g, Na2HPO4:0.008g, SrCl2:0.034g, H3BO3:0.022g, Na2SiO3:0.004g, NaF:0.0024g, Agar:15.0g, pH:7.4-7.8(25℃) |
| Growth conditions | 30 ℃; 24-48 hours; aerobic; |
| Subculture procedure | Prepare 1-2 fresh agar plates with typical bacterial colonies; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Transfer 0.5mL of liquid culture medium into a freeze-drying tube, dissolve it thoroughly, and then transfer it back into the liquid test tube, mixing well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Cultivate under the above conditions, and the strain can be used once it grows. |
| Storage conditions | 2-8 ℃ |
| Safety level | 1 |
| morphology | The colony diameter is 0.5-1mm, circular, with neat edges, opaque, gray white on the front, raised in the middle, smooth on the surface, moist texture, G - (red), Bacillus, purity: pure |
| Sharing mode | Public welfare sharing |
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