BeNa Culture Collection
info@bncc.com
| Culture medium | BNCC360798 |
| Description | Colombia Agar Chocolate Plate |
| Composition | Special Peptone:23.0g, Soluble Starch:1.0g, NaCl:5.0g, Agar:20.0g, Defibrinated Sheep Blood:5%, pH:7.3±0.2(25℃) |
| Growth conditions | 37 ℃; 48-72h；5%CO2； |
| Subculture procedure | ① Prepare 2 plates; ② Open the safety cabinet, burn the top with an alcohol lamp, quickly drip sterile water to break it, and then use tweezers to crush it; ③ Take 0.5mL of sterile water and pour it into the freeze-drying tube. After fully dissolving, mix well; ④ Transfer 0.2mL of bacterial suspension into a Agar plate, apply evenly, and repeat twice to obtain two plates; ⑤ Place all the plates under the above cultivation conditions, and once the strains grow, they can be used. |
| Storage conditions | 2-8 ℃ |
| Safety level | 2 |
| morphology | The colony diameter is 1-2mm, circular, with neat edges, opaque, gray white front, flat shape, smooth surface, bright surface, moist texture, easy to pick up, G - (red), cocci, purity: pure |
| Sharing mode | Public welfare sharing |
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