BeNa Culture Collection
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| Culture medium | BNCC353538 |
| Description | Liquid thioglycolate culture medium |
| Composition | Tryptone(Pancreatic Digest):15.0g, Yeast Extract Powder:5.0g, Glucose:5.0g, Sodium Thioglycolate(C2H3NaO2S):0.5g, L-Cystine:0.5g, NaCl:2.5g, Resazurin:0.001g, Agar:0.75g, pH:7.1±0.2(25℃) |
| Growth conditions | 37 ℃; 3-7 days; anaerobic; |
| Subculture procedure | ① Dissolve the Frozen vial tube in a water bath (bacteria 37 ℃) and shake rapidly for dissolution; ② Wipe the outer wall of the Frozen vial with an alcohol swab for disinfection, and then transfer it to a safe cabinet for operation; ③ Unscrew the tube cap, aspirate all the dissolved solution, and transfer it into a test tube (for anaerobic bacteria cultivation, the culture medium should be placed in an anaerobic environment for 24 hours to remove oxygen); ④ Transfer to the above cultivation conditions for cultivation. |
| Storage conditions | -80 ℃ |
| Safety level | 1 |
| morphology | The liquid culture medium is slightly turbid, with flocculent, G - (red), and oval shaped bacteria |
| Sharing mode | Public welfare sharing |
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